Superresolution, Multi-photon and Correlative Fluorescence / Atomic Force Microscopy of Nano- and Bio-Materials

Event Sponsor: 
Materials Science Seminar
Start Date: 
Sep 21 2015 - 4:00pm
Building 241/Room C201
Argonne National Laboratory
Steve Smith
Speaker(s) Title: 
South Dakota School of Mines

Our group at SDSMT focuses on spectroscopy and imaging of nano- and bio-materials. A number of specific applications of imaging methods to select nano- and bio-materials will be discussed, these include: Photo-activated Localization microscopy of single Carbohydrate Binding Modules (CBMs) on nano-crystalline cellulose, multi-photon imaging of fluorescent reporters of gene expression in soy bean root nodules, spectroscopic imaging of the surface plasmon polariton enhanced spectral upconversion in rare-earth doped nano-crystals, and simultaneous fluorescence and atomic force microscope imaging of clathrin mediated endocytotic vesicles in unroofed fixed cells. Time permitting, I will discuss our development of a lattice light-sheet microscope for super resolution, near-video rate volumetric imaging in living cells.