X-ray Microscopy of Extended 3D Objects: Scaling Towards the Future

PI Chris Jacobsen, Argonne National Laboratory and Northwestern University
Jacobsen ADSP2020

The approach to beyond-pure-projection x-ray image reconstruction methods involves modeling the object of study, carrying out multi-slice propagation, and using numerical optimization to recreate actual detected intensities at each illumination angle. Credit: Ming Du and Chris Jacobsen, Argonne National Laboratory

The 3D x-ray microscopy of frozen hydrated biological specimens is currently approaching a limit to specimen thickness, the surpassing of which violates the pure projection approximation (PPA) needed for standard tomographic imaging. Sufficient understanding of the underlying problem has enabled development of a novel approach to beyond-pure-projection x-ray image construction utilizing powerful—but computationally demanding—methods. This project aims to scale up these methods to meet the challenge of high-resolution x-ray imaging beyond the PPA, benefitting not just cell and brain imaging but the full range of future nanoscale imaging activities at DOE light sources.

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